C3h10细胞用什么培养基培养
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C3h10细胞用什么培养基培养
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Web前往丁香实验. 丁香园网友kj7156084的 培养 方法：. 本室的PC－3来自上海细胞生物所，种是ATCC的。. 以10％FBS＋1640或F12培养，0.25%胰酶消化。. 塑料瓶 或玻璃瓶都可以，当然，前者效果较好。. 复苏时最好用塑料瓶。. 5－7天传一次（1：2），2－3天换一次液 … Web假期太快结束，又到了复苏细胞开始实验的环节了 ，以下是整理的一些复苏细胞的注意事项： 1. 细胞储存在液氮中，温度达-196℃，理论上储存时间是无限的。只要一直在液氮，冻存没有问题，那么复苏也不会出现很大的…
Web一般使用的是新生牛血清培养，用胎牛血清生长速度会加快 ，容易使细胞分化；. 传代时铺板需均匀 ，不然会导致分化不均匀，分化比例低。. 培养好3T3-L1细胞后，就可以开始准备成脂诱导了。. 我们先来了解一下 成脂分化 是一个怎样的过程：. 前脂肪细胞是 ... WebJun 21, 2004 · Based on earlier reports ( 19 ), § we tested the possibility that BMP4 can induce C3H10T1/2 cells to commit to the adipocyte lineage. To generate BMP4-conditioned medium, human embryonic kidney 293T cells were stably transfected with a BMP4 …
WebDec 10, 2024 · Brown adipose tissue (BAT) and beige adipose tissue dissipate metabolic energy and mediate nonshivering thermogenesis, thereby boosting energy expenditure. Increasing the browning of BAT and beige adipose tissue is expected to be a promising strategy for combatting obesity. Through phenotype screening of C3H10-T1/2 … WebDec 10, 2024 · Through phenotype screening of C3H10-T1/2 mesenchymal stem cells, diphyllin was identified as a promising molecule in promoting brown adipocyte differentiation. In vitro studies revealed that diphyllin promoted C3H10-T1/2 cell and primary brown/beige preadipocyte differentiation and thermogenesis, which resulted increased energy …
WebDec 15, 2003 · In summary, the murine mesenchymal stem cell line C3H10T1/2 was induced to follow an endochondral sequence of chondrogenic and osteogenic differentiation dependent on both dose and continual presence of BMP-7 and enhanced by a nutrient-rich media. Our preliminary results suggest that the induction of osteogenesis is dependent …
WebOct 27, 2024 · pKa is an acid dissociation constant used to describe the acidity of a particular molecule. Its value is directly related to the structure of the given compound. The constant changes depending on the solvent the compound is used in. Typically, organic chemists compare the various values from their determination in water, DMSO and the … hayland horse simWebButane (/ ˈ b juː t eɪ n /) or n-butane is an alkane with the formula C 4 H 10.Butane is a highly flammable, colorless, easily liquefied gas that quickly vaporizes at room temperature and pressure. The name butane comes from the root but-(from butyric acid, named after the Greek word for butter) and the suffix -ane.It was discovered in crude petroleum in 1864 … hayland glovesWebATCC mesenchymal stem cells c3h10 t1 2. 94 stars, based on 1 article reviews. Price from $9.99 to $1999.99. mesenchymal stem cells c3h10 t1 2 - by Bioz Stars , 2024-03. 94 / 100 stars. hay land for leaseWeb将C3H10 T1/2细胞以30%融合度铺在24孔板里，病毒处理24h后加入成骨诱导培养基 (含DMEM，10%胎牛血清，1%青/链霉素，10mmol/L β磷酸甘油及50mg/ml维生素C) 和BMP9条件培养基，在第14天进行茜素红染色：弃去培养基，PBS洗2次，0.05%戊二醛固 … hayland ltd fredericton nbWebSep 15, 2015 · Micromass cultures of ATDC5 cells or C3H10 cells infected with siATF6 adenovirus or control adenovirus (CTR) were treated with BMP2 for various time points. As shown in Fig. 6 , knockdown of ATF6 obviously abolished the collagen II (Fig. 6 b, f), collagen X (Fig. 6 c, g), and MMP13 (Fig. 6 d, h) induction during chondrocyte … hayland properties ltdWebTo write the empirical, molecular, and structural formula for Ethane (C2H6), we'll start with the molecular formula.The molecular formula shows us the number... hayland properties frederictonWebDec 10, 2024 · C3H10-T1/2 cells were a gift kindly given by Jiqiu Wang, Ruijin Hospital. All the cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% FBS (Gibco), 0.1 mg/ml streptomycin (Invitrogen) and 100 U/ml penicillin at … bottin cma